Artificial Intelligence
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Why do we not use an endogenous control in conventional pcr


Endogenous controls are used in quantitative PCR (qPCR) assays, not in conventional PCR. This is because the purpose of endogenous controls is to normalize the data in qPCR, which is used to measure gene expression levels. Conventional PCR, on the other hand, is used primarily for the qualitative detection of specific DNA sequences and does not require normalization of the data.