In research, the insertion of genes into *Legionella* species can be an important method for studying gene function, pathogenicity, and various cellular processes. *Legionella* is a genus of bacteria that includes *Legionella pneumophila*, the causative agent of Legionnaires' disease. Gene insertion techniques in *Legionella* may involve various genetic engineering methods, such as:
1. **Gene Cloning**: Involves the insertion of a DNA fragment


